NADH

NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at 334 nm (Hg), 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at 365 nm (Hg)

Formula: C₂₁H₂₇N₇O₁₄P₂Na₂

pH value in water: 7.5 ± 0.5 (100 mg/ml)

Packaged by solid weight.

Amorphous powder

Activator: Mg²⁺ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

Store at 2 to 8 °C. (Store dry!)

Purity: 98% β-NADH-Na₂ based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A₂₆₀ nm-A₃₄₀ nm: <2.40
Organic solvents: <4% (GC)

For life science research only. Not for use in diagnostic procedures.