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10165875001

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

Name: Glucose-6-Phosphate Dehydrogenase (G6P-DH)
Brand: ROCHE

from Leuconostoc mesenteroides

Synonym(s):

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

UNSPSC Code:

12352204

EC Number:

1.1.1.49 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)

Biological source:

bacterial (Leuconostoc mesenteroides)

Concentration:

≥0.1-1.0 % (w/w)

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Properties

biological source

bacterial (Leuconostoc mesenteroides)

form

solution, suspension

specific activity

~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)

mol wt

dimer 110 kDa

packaging

pkg of 1 mL (1,000 U)

manufacturer/tradename

Roche

concentration

≥0.1-1.0 % (w/w)

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-8.5(maximal activity at 7.8)

solubility

water: miscible

NCBI accession no.

AAA25265

UniProt accession no.

P11411

application(s)

life science and biopharma

foreign activity

6-PGDH <0.001%, CK <0.001%, GR <0.01%, HK <0.05%, NADH oxidase <0.02%, PGI <0.01%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Application

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Component of cofactor recycling systems for NADPH.

Biochem/physiol Actions

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Physical form

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Preparation Note

Activator: HCO^3- (≤ 0.3 M) activates slightly.

Analysis Note

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)