biological source
bacterial (Leuconostoc mesenteroides)
form
solution, suspension
specific activity
~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
mol wt
dimer 110 kDa
packaging
pkg of 1 mL (1,000 U)
manufacturer/tradename
Roche
concentration
≥0.1-1.0 % (w/w)
technique(s)
activity assay: suitable
color
white
optimum pH
7.0-8.5(maximal activity at 7.8)
solubility
water: miscible
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
6-PGDH <0.001%, CK <0.001%, GR <0.01%, HK <0.05%, NADH oxidase <0.02%, PGI <0.01%
shipped in
wet ice
storage temp.
2-8°C
General description
Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1
Application
Component of cofactor recycling systems for NADPH.
It was used in enzymatic determination of C6 phosphorylation of glycogen.
Biochem/physiol Actions
Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (Km = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (Km = 7.4 μM; relative rate 1.0) or NAD (Km = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.
Physical form
Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6
Preparation Note
Activator: HCO3- (≤ 0.3 M) activates slightly.
Analysis Note
Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH
Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme
Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.
Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10165875001 | 04061838668417 |