biological source
bovine liver
form
lyophilized
specific activity
10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
packaging
pkg of 3,000 U
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
white
optimum pH
8
solubility
water: 20 mg/mL, colorless
suitability
suitable for UV spectrophotometry and general use
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.00500%, LDH <0.00500%, MDH <0.00500%
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... GLUD1(281785)
General description
Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.
GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination.
Application
Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.
Analysis Note
Contaminants: <0.005% ADH, LDH, and MDH, each
Other Notes
For life science research only. Not for use in diagnostic procedures.
The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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