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10220647001

Roche

GTP-γ-S

Name: GTP-γ-S
Brand: ROCHE

Tetralithium salt

Synonym(s):

GTP-γ-S, GTP

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

Assay:

87% (HPLC)

Form:

solid

Solubility:

water: soluble

Storage temp.:

−20°C (−15°C to −25°C)

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Properties

description

C₁₀H₁₂N₅O₁₃P₃SLi₄

assay

87% (HPLC)

form

solid

mol wt

M_r 539.2 (GTP-γ-S), M_r 563.0 (GTP-γ-S-Li₄)

composition

GDP, ≤12% HPLC , GMP, ≤1% HPLC , GTP, ≤2% HPLC

packaging

pkg of 10 mg

manufacturer/tradename

Roche

storage condition

protect from light

concentration

97%

technique(s)

activity assay: suitable

color

white

solubility

water: soluble

storage temp.

−20°C (−15°C to −25°C)

Description

General description

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Application

GTP-γ-S has been used:

in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants

Biochem/physiol Actions

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

Preparation Note

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.

Analysis Note

Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:

approx. 10 mg/ml double-dist. water
dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c