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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Assay:
87% (HPLC)
Form:
solid
Solubility:
water: soluble
Storage temp.:
−20°C (−15°C to −25°C)
description
C10H12N5O13P3SLi4
assay
87% (HPLC)
form
solid
mol wt
Mr 539.2 (GTP-γ-S), Mr 563.0 (GTP-γ-S-Li4)
composition
GDP, ≤12% HPLC , GMP, ≤1% HPLC , GTP, ≤2% HPLC
packaging
pkg of 10 mg
manufacturer/tradename
Roche
storage condition
protect from light
concentration
97%
technique(s)
activity assay: suitable
color
white
solubility
water: soluble
storage temp.
−20°C (−15°C to −25°C)
General description
Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.
Application
GTP-γ-S has been used:
- in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
- in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
- to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants
Biochem/physiol Actions
Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),
Preparation Note
Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.
Analysis Note
Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:
Conditions:
- approx. 10 mg/ml double-dist. water
- dilution: 9.8 ml double-dist. water + 0.2 ml sample.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10220647001 | 04061838669322 |