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About This Item
Empirical Formula (Hill Notation):
C21H25NO5
CAS Number:
Molecular Weight:
371.43
PubChem Substance ID:
UNSPSC Code:
12352207
Beilstein/REAXYS Number:
2822892
MDL number:
NACRES:
NA.54
Form:
solution
description
N-methyl-N-deacetyl-colchicine
sterility
sterile; 0.2 μm filtered
form
solution
packaging
pkg of 20 mL (10 μg/ml)
manufacturer/tradename
Roche
technique(s)
blocking: suitable
impurities
microbial, tested
solubility
water: miscible
storage temp.
2-8°C
SMILES string
CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
InChI
1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
InChI key
NNJPGOLRFBJNIW-HNNXBMFYSA-N
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Related Categories
General description
Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.
Application
Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.
Biochem/physiol Actions
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Physical form
Solution (10 μg/ml), filtered through 0.2 μm pore size membrane.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
涉药品监管产品
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Johannes Bloehdorn et al.
Nature communications, 12(1), 5395-5395 (2021-09-15)
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Naiara Zoccal Saraiva et al.
Cloning and stem cells, 11(1), 141-152 (2009-02-20)
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to
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The human RB1 gene is imprinted due to integration of the PPP1R26P1 pseudogene into intron 2. PPP1R26P1 harbors the gametic differentially methylated region of the RB1 gene, CpG85, which is methylated in the female germ line. The paternally unmethylated CpG85
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eLife, 5, e11402-e11402 (2016-02-18)
Phosphorylation of histone H3 threonine 118 (H3 T118ph) weakens histone DNA-contacts, disrupting the nucleosome structure. We show that Aurora-A mediated H3 T118ph occurs at pericentromeres and chromosome arms during prophase and is lost upon chromosome alignment. Expression of H3 T118E
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The Journal of cell biology, 220(4) (2021-02-24)
Chromosomal instability (CIN) is a hallmark of many cancers. Restricting the localization of centromeric histone H3 variant CENP-A to centromeres prevents CIN. CENP-A overexpression (OE) and mislocalization have been observed in cancers and correlate with poor prognosis; however, the molecular
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10295892001 | 04061838670083 |
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