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Merck
CN

10775835001

Roche

Bovine Serum Albumin

98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin

Synonym(s):

BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.54
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Product Name

Bovine Serum Albumin, 98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin

biological source

bovine

assay

98.5% (electrophoresis)

form

lyophilized

mol wt

Mr 68 kDa

packaging

pkg of 50 g

manufacturer/tradename

Roche

origin

USA origin

technique(s)

immunofluorescence: suitable
ligand binding assay: suitable

impurities

Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... ALB(280717)

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Analysis Note

Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

Application

Bovine Serum Albumin, Fraction V, fatty acid free is used:
  • in lipid research to study lipid metabolism in biological systems
  • to identify transport mechanisms in living organisms
  • to study ligand binding
  • to investigate ionic binding
  • in the preparation of solutions during isolation of cardiomyocytes
  • as a blocking solution to incubate embryos for immunofluorescence
  • in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

Biochem/physiol Actions

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.

Features and Benefits

  • Free from fatty acids according to current quality control procedures (suitable for lipid research)
  • High quality
  • Low content of heavy metal ions
  • Long-term stability

General description

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
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Putnam F
The Plasma Proteins: Structure, Function, and Genetic Control, 5 (2012)
Examination of the Interaction between a Membrane Active Peptide
and Artificial Bilayers by Dual Polarisation Interferometry
Payne JAE, et al.
Bio-protocol, 7(1), 1-12 (2017)
Relief of endoplasmic reticulum stress enhances DNA damage repair and improves development of pre-implantation embryos
Dicks N, et al.
PLoS ONE, 12(11), e0187717-e0187717 (2017)
Tiziana Caputo et al.
Cellular and molecular life sciences : CMLS, 78(1), 227-247 (2020-03-12)
Chronic inflammation that affects primarily metabolic organs, such as white adipose tissue (WAT), is considered as a major cause of human obesity-associated co-morbidities. However, the molecular mechanisms initiating this inflammation in WAT are poorly understood. By combining transcriptomics, ChIP-seq and
Geronimo Matteo et al.
Endocrinology, 162(6) (2021-03-12)
Human studies consistently show an association between exposure to persistent organic pollutants, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, aka "dioxin"), and increased diabetes risk. We previously showed that a single high-dose TCDD exposure (20 µg/kg) decreased plasma insulin levels in male and female

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