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Roche

Fibronectin

from human plasma

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Synonym(s):
Fibronectin
MDL number:
MFCD00131062

biological source

human plasma

Quality Level

100

sterility

non-sterile; 0.2 μm filtered

Assay

95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

mol wt

440 kDa

packaging

pkg of 5 mg

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02751

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Specificity

Active on most mammalian cells

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Testing Method

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Physical form

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Preparation Note

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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