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10884308001

Roche

CPRG

Name: CPRG
Brand: ROCHE

Chlorophenol red-β-D-galactopyranoside

Synonym(s):

Chlorophenol Red-β-D-galactopyranoside, chlorophenol red-β-d-galactopyranoside, CPRG, Chlorophenol Red-β-D-galactoside

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₂₂Cl₂O₁₀S

CAS Number:

99792-79-7

Molecular Weight:

585.41

UNSPSC Code:

12352204

PubChem Substance ID:

329749219

MDL number:

MFCD05863726

NACRES:

NA.54

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Properties

assay

96% (HPLC)

form

powder

packaging

pkg of 250 mg

manufacturer/tradename

Roche

storage condition

protect from light

pH range

6-8

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

SMILES string

OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1

InChI key

YOUWVNCQJOMMEU-AETRGLENSA-N

Description

General description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Application

CPRG has been used as a substrate for β-galactosidase assay.

Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

Preparation Note

Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

Analysis Note

Contaminant: <0.1% chlorophenol red

Other Notes

CPRG is an approximately 1:1 mixture of the isomers.

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

No data available

flash_point_c