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Roche

Glycogen

from mussels

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Synonym(s):
Glycogen, glycogen

Quality Level

100

form

solution

packaging

pkg of 1 mL (20 mg)

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

Application

This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.

Features and Benefits

Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.

Contents
Aqueous solution, 20 mg/ml

Quality

Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Richard C Edmunds et al.
Journal of biomolecular techniques : JBT, 31(4), 125-150 (2020-10-27)
Unfiltered and filtered water samples can be used to collect environmental DNA (eDNA). We developed the novel "Preserve, Precipitate, Lyse, Precipitate, Purify" (PPLPP) workflow to efficiently extract eDNA from Longmire's preserved unfiltered and filtered water samples (44-100% recovery). The PPLPP
Hanwen Gu et al.
Poultry science, 100(9), 101321-101321 (2021-07-24)
Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies as they encode for
Ming Wang et al.
Aging cell, 19(6), e13147-e13147 (2020-05-01)
Progerin accumulation disrupts nuclear lamina integrity and causes nuclear structure abnormalities, leading to premature aging, that is, Hutchinson-Gilford progeria syndrome (HGPS). The roles of nuclear subcompartments, such as PML nuclear bodies (PML NBs), in HGPS pathogenesis, are unclear. Here, we
Sudhir Gopal Tattikota et al.
eLife, 9 (2020-05-13)
Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing
Xiwen Cheng et al.
The Journal of biological chemistry, 288(41), 29746-29759 (2013-08-30)
The promyelocytic leukemia protein is a well known tumor suppressor, but its role in metabolism is largely unknown. Mice with a deletion in the gene for PML (KO mice) exhibit altered gene expression in liver, adipose tissue, and skeletal muscle
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