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Merck
CN

10901393001

Roche

糖原

from mussels

别名:

糖原

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关于此项目

UNSPSC代码:
41116100
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质量水平

表单

solution

包装

pkg of 1 mL (20 mg)

制造商/商品名称

Roche

储存温度

−20°C

应用

该制剂可用作核酸(DNA或RNA)沉淀的载体。作为惰性材料,它可以代替tRNA或超声处理的DNA。
20 μg糖原(1 μl溶液)可以从1 ml的体积中沉淀出pg量的DNA或RNA。
在一个典型实验中,将5 pg [3H]标记的小牛胸腺DNA溶解于含1 mM EDTA、0.4 M LiCl的500 μl 10 mM Tris-HCl(pH 8.0)中。加入1 μl糖原溶液(20 μg糖原)作为载体,然后在-15至-25 °C下用1.2 ml乙醇沉淀,并在-15至-25 °C下保存3小时。 离心(12 000×g 10分钟)后,总放射性存在于沉淀物中。不添加糖原,DNA不会沉淀。

特点和优势

糖原具有特殊的分子生物学性质,是核酸制剂中的惰性载体。

内容
水溶液,20 mg/ml

分析说明

根据现行质量控制程序,检测是否存在核酸内切酶、切割活性、核酸外切酶、核糖核酸酶、核酸和蛋白酶。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

does not flash

闪点(°C)

does not flash


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Hanwen Gu et al.
Poultry science, 100(9), 101321-101321 (2021-07-24)
Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies as they encode for
Ming Wang et al.
Aging cell, 19(6), e13147-e13147 (2020-05-01)
Progerin accumulation disrupts nuclear lamina integrity and causes nuclear structure abnormalities, leading to premature aging, that is, Hutchinson-Gilford progeria syndrome (HGPS). The roles of nuclear subcompartments, such as PML nuclear bodies (PML NBs), in HGPS pathogenesis, are unclear. Here, we
Gabriele Girelli et al.
Nature biotechnology, 38(10), 1184-1193 (2020-05-27)
With the exception of lamina-associated domains, the radial organization of chromatin in mammalian cells remains largely unexplored. Here we describe genomic loci positioning by sequencing (GPSeq), a genome-wide method for inferring distances to the nuclear lamina all along the nuclear
Yunbo Qiao et al.
Nature communications, 11(1), 2653-2653 (2020-05-29)
The transcriptome of the preimplantation mouse embryo has been previously annotated by short-read sequencing, with limited coverage and accuracy. Here we utilize a low-cell number transcriptome based on the Smart-seq2 method to perform long-read sequencing. Our analysis describes additional novel
Drew Weissman et al.
Methods in molecular biology (Clifton, N.J.), 969, 43-54 (2013-01-09)
In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA. While the reaction can yield large quantities of RNA, it contains impurities due to various unwanted activities of the polymerases.

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