form
solution
packaging
pkg of 1 mL (20 mg)
manufacturer/tradename
Roche
storage temp.
−20°C
Application
该制剂可用作核酸（DNA或RNA）沉淀的载体。作为惰性材料，它可以代替tRNA或超声处理的DNA。
20 μg糖原（1 μl溶液）可以从1 ml的体积中沉淀出pg量的DNA或RNA。
在一个典型实验中，将5 pg [3H]标记的小牛胸腺DNA溶解于含1 mM EDTA、0.4 M LiCl的500 μl 10 mM Tris-HCl（pH 8.0）中。加入1 μl糖原溶液（20 μg糖原）作为载体，然后在-15至-25 °C下用1.2 ml乙醇沉淀，并在-15至-25 °C下保存3小时。 离心（12 000×g 10分钟）后，总放射性存在于沉淀物中。不添加糖原，DNA不会沉淀。
20 μg糖原（1 μl溶液）可以从1 ml的体积中沉淀出pg量的DNA或RNA。
在一个典型实验中，将5 pg [3H]标记的小牛胸腺DNA溶解于含1 mM EDTA、0.4 M LiCl的500 μl 10 mM Tris-HCl（pH 8.0）中。加入1 μl糖原溶液（20 μg糖原）作为载体，然后在-15至-25 °C下用1.2 ml乙醇沉淀，并在-15至-25 °C下保存3小时。 离心（12 000×g 10分钟）后，总放射性存在于沉淀物中。不添加糖原，DNA不会沉淀。
Features and Benefits
糖原具有特殊的分子生物学性质，是核酸制剂中的惰性载体。
内容
水溶液，20 mg/ml
内容
水溶液，20 mg/ml
Analysis Note
根据现行质量控制程序，检测是否存在核酸内切酶、切割活性、核酸外切酶、核糖核酸酶、核酸和蛋白酶。
Other Notes
仅用于生命科学研究。不可用于诊断。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
Gabriele Girelli et al.
Nature biotechnology, 38(10), 1184-1193 (2020-05-27)
With the exception of lamina-associated domains, the radial organization of chromatin in mammalian cells remains largely unexplored. Here we describe genomic loci positioning by sequencing (GPSeq), a genome-wide method for inferring distances to the nuclear lamina all along the nuclear
Yunbo Qiao et al.
Nature communications, 11(1), 2653-2653 (2020-05-29)
The transcriptome of the preimplantation mouse embryo has been previously annotated by short-read sequencing, with limited coverage and accuracy. Here we utilize a low-cell number transcriptome based on the Smart-seq2 method to perform long-read sequencing. Our analysis describes additional novel
Xiwen Cheng et al.
The Journal of biological chemistry, 288(41), 29746-29759 (2013-08-30)
The promyelocytic leukemia protein is a well known tumor suppressor, but its role in metabolism is largely unknown. Mice with a deletion in the gene for PML (KO mice) exhibit altered gene expression in liver, adipose tissue, and skeletal muscle
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10901393001 | 04061837684548 |