Key Documents

View All Documentation

10988537001

Roche

7-Deaza-2′-deoxy-guanosine-5′-triphosphate

Name: 7-Deaza-2′-deoxy-guanosine-5′-triphosphate
Brand: ROCHE

95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM

Synonym(s):

7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt, −N⁷-dGTP, 7-Deaza-dGTP

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₁₁H₁₇N₄O₁₃P₃

CAS Number:

101515-08-6

Molecular Weight:

506.19

UNSPSC Code:

41116100

PubChem Substance ID:

329749263

MDL number:

MFCD00171614

NACRES:

NA.54

Form:

solution

Storage temp.:

−20°C

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

form

solution

composition

7-Deaza-dGDP, ≤4% HPLC , 7-Deaza-dGTP, 95% HPLC

packaging

pkg of 200 μL (10 mM, 2 μmol)

manufacturer/tradename

Roche

concentration

10 mM

storage temp.

−20°C

SMILES string

NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1

InChI

1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)

InChI key

DLLXAZJTLIUPAI-UHFFFAOYSA-N

Description

General description

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.

The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.

Application

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.PMID 12216071
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.PMID 21831882 PMID 23413259
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).

Preparation Note

Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.

Analysis Note

Absorption: 25 A₂₆₀ units of product correspond to 1 mg.

Absorbance determination: A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Other Notes

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library