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Merck
CN

11051458001

Roche

dCTP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

Synonym(s):

dCTP, 2′-deoxy-cytidine-5′-triphosphate, deoxy-cytidine-5′-triphosphate, 2′-

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About This Item

Empirical Formula (Hill Notation):
C9H16N3O13P3
Molecular Weight:
467.16
UNSPSC Code:
41116100
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Quality Level

description

Lithium salt

Assay

98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable
DNA sequencing: suitable

storage temp.

−20°C

General description

dCTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dCTP solution in water (pH 7), 100 mM, lithium salt.

Application

Use dCTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Preparation Note

This product is stable at -15 to -25°C until the expiration date printed on the label.
Note: Avoid repeated freezing and thawing. Store in aliquots.

Analysis Note

Typical analysis:
  • ≥98% dCTP (HPLC), ≤1.5% dCDP (HPLC), ≤0.5% dCMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Other Notes

1 μmol dCTP = 9.6 A272 units
For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


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