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11051466001

Roche

dGTP

Name: dGTP
Brand: ROCHE

=98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing

Synonym(s):

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 2′-deoxy-guanosine-5′-triphosphate, deoxy-guanosine-5′-triphosphate, 2′-, dGTP-Na₃

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₃N₅Na₃O₁₃P₃

CAS Number:

93919-41-6

Molecular Weight:

573.13

UNSPSC Code:

41116100

PubChem Substance ID:

329749301

MDL number:

MFCD00080337

NACRES:

NA.54

Assay:

98%

Form:

solution

Storage temp.:

−20°C

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Properties

description

Lithium salt

assay

98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol; 100 mM)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable, DNA sequencing: suitable, DNA synthesis: suitable, PCR: suitable

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1

InChI

1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1

InChI key

IWGGLKOTEOCWQP-BIHLCPNHSA-K

Description

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Analysis Note

Typical analysis:

≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
DNase activity negative, according to the current Quality Control procedures
Suitability for PCR

Other Notes

1 μmol dGTP = 13.7 A₂₅₂ units

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

nucleotide sets (all 4 nucleotides in separate vials)
ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

kits (dNTPs, buffer, and polymerase in separate vials)
ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available

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Global Trade Item Number

SKU	GTIN
11051466001	04061838697523