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11074547001

Roche

Insulin-Transferrin-Sodium Selenite Supplement

suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)

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Synonym(s):
ITS

biological source

human serum (transferrin)
synthetic (sodium selenite)

Quality Level

100

recombinant

expressed in human cells

sterility

non-sterile; 0.2 μm filtered

Assay

>98% (Transferrin, SDS-PAGE)
>99% (Insulin, HPLC)
>99% (Sodium selenite)

form

lyophilized

packaging

pkg of 50 mg (for 5 l medium)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

solubility

water: miscible

UniProt accession no.

P01317
P02786

storage temp.

2-8°C

Gene Information

bovine ... INS(280829)
human ... TFRC(7037)

Related Categories

General description

The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.

Application

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.

Preparation Note

Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)

Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Imran Khan et al.
Theriogenology, 152, 147-155 (2020-05-16)
Hypothermic storage of gametes and embryos at 4 °C can be used as an alternative to cryopreservation, but hypothermic preservation can maintain embryo viability for a short duration only. This study investigated the effect of insulin-transferrin-sodium selenite (ITS) in embryo culture
Jyoti Motwani et al.
Epigenomics, 13(8), 577-598 (2021-03-31)
Aims & objectives: The aim of this study was to investigate the role of DNA methylation in invasiveness in melanoma cells. Materials & methods: The authors carried out genome-wide transcriptome (RNA sequencing) and reduced representation bisulfite sequencing methylome profiling between noninvasive (n = 4)
K H Chua et al.
European cells & materials, 9, 58-67 (2005-06-18)
This study was to investigate the effects of insulin-transferrin-selenium (ITS) on the proliferation and quantitative gene expression of adult human nasal septum chondrocytes in monolayer culture expansion and the formation of tissue engineered hyaline cartilage. Effects of ITS on human
María Virumbrales-Muñoz et al.
Lab on a chip, 20(23), 4420-4432 (2020-10-27)
Clear cell renal cell carcinoma (ccRCC) is a common genitourinary cancer associated with the development of abnormal tumor angiogenesis. Although multiple anti-angiogenic therapies have been developed, responses to individual treatment are highly variable between patients. Thus, the use of one-patient
Catherine Jane Messner et al.
International journal of molecular sciences, 22(18) (2021-09-29)
Liver fibrosis is characterized by the accumulation of extracellular matrix (ECM) resulting in the formation of fibrous scars. In the clinic, liver biopsies are the standard diagnostic method despite the potential for clinical complications. miRNAs are single-stranded, non-coding RNAs that
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