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11243217001

Roche

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Synonym(s):

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

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About This Item

CAS Number:
114956-81-9
UNSPSC Code:
12352207
MDL number:
MFCD00081739
NACRES:
NA.54
Biological source:
mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Mol wt:
900 kDa
Assay:
≥90% (SDS-PAGE)
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biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

sterility

sterile; sterile-filtered

assay

≥90% (SDS-PAGE)

form

solution

mol wt

900 kDa

packaging

pkg of 1 mg (2 ml)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q61292

storage temp.

−20°C

Gene Information

mouse ... Lamb2(16779)

General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Physical form

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane

Preparation Note

Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Analysis Note

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
91414200
75215900
74228300
62867700
44741900

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Protocols

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

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The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D 细胞培养工具和技术

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Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
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Global Trade Item Number

SKUGTIN
1124321700104061838258984