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11363905910

Roche

Digoxigenin-11-ddUTP

solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

Synonym(s):

digoxigenin

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About This Item

UNSPSC Code:
41116100
NACRES:
NA.54
Assay:
85% (HPLC)
Form:
solution
Storage temp.:
−20°C
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assay

85% (HPLC)

form

solution

mol wt

(Molecular weight: Mr = 1074.7 (DIG-11-ddUTP-Li4))

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Application

Digoxigenin-11-ddUTP has been used:
  • for labeling of DNA fragments in linker histone binding assay
  • for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions

Analysis Note

Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C43H61N4O20P3Li4

Other Notes

For life science research only. Not for use in diagnostic procedures.


Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash



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