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11383221001

Roche

BCIP

solution, >95% (HPLC), pkg of 3 mL (150 mg)

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Synonym(s):
4-toluidine salt

description

4-toluidine salt

Quality Level

Assay

>95% (HPLC)

form

solution

packaging

pkg of 3 mL (150 mg)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

Use BCIP in alkaline phosphatase detection:
  • Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque hybridization
  • In situ hybridization

Quality

Function test: incubation with AP

Principle

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Physical form

Solution (50 mg/ml) in dimethylformamide (DMF)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Dermal - Eye Irrit. 2 - Flam. Liq. 3 Inhalation - Repr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Regulatory Information

危险化学品

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Jinlong Chen et al.
Dental materials : official publication of the Academy of Dental Materials, 36(10), 1303-1313 (2020-07-12)
Compared to autologous bone grafts, allogeneic bone grafts integrate slowly, which can adversely affect clinical outcomes. Here, our goal was to understand the molecular mechanisms underlying graft incorporation, and then test clinically feasible methods to accelerate this process. Wild-type and
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Frontiers in cell and developmental biology, 9, 595744-595744 (2021-04-20)
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 (Shh brain enhancer 2) in mouse markedly reduced Shh expression within the embryonic brain specifically
Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Ewa Pocheć et al.
Acta biochimica Polonica, 62(2), 323-328 (2015-06-23)
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes
Surendra Singh Patel et al.
The Journal of experimental biology, 223(Pt 8) (2020-02-27)
Eisenia fetida, the common vermicomposting earthworm, shows robust regeneration of posterior segments removed by amputation. During the period of regeneration, the newly formed tissue initially contains only undifferentiated cells but subsequently differentiates into a variety of cell types including muscle

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