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11427857910

Roche

Fluorescein-12-UTP

Name: Fluorescein-12-UTP
Brand: ROCHE

≥85% (HPLC), solution

Synonym(s):

Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)

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About This Item

Empirical Formula (Hill Notation):

C₃₉H₄₁N₄O₂₂P₃

CAS Number:

134367-01-4

Molecular Weight:

1010.68

UNSPSC Code:

41116100

MDL number:

MFCD00866062

NACRES:

NA.54

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Properties

assay

≥85% (HPLC)

form

solution

mol wt

1034.4

packaging

pkg of 25 μL (250 nmol; 10 mM)

manufacturer/tradename

Roche

storage temp.

−20°C

Description

General description

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).

Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C₃₉H₃₇N₄O₂₂P₃Li₄.

Application

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.

For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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