biological source
bovine (calf) intestine
assay
>95% (HPLC)
form
lyophilized (clear, colorless solution after reconstitution)
usage
sufficient for ≤5 labeling reactions
specific activity
8 units/μg protein (At 25 °C with 4-nitro-phenylphosphate as substrate (pH 9.8)
)
packaging
pkg of 10 mg (approx. 40 mg lyophilizate)
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
The quantity of enzyme is sufficient to conjugate approx. 2 mg immunoglobulin G (IgG). Roche recommends dividing the total quantity to be conjugated into 5 portions; each portion provides 0.3 to 0.5 ml of conjugate from approx. 0.35 mg IgG; this quantity can be diluted 1:4,000 to 1:10,000 for ELISA applications.
Contents
Alkaline phosphatase from calf intestine lyophilizate from 0.5 ml.
The quantity of enzyme is sufficient to conjugate approx. 2 mg immunoglobulin G (IgG). Roche recommends dividing the total quantity to be conjugated into 5 portions; each portion provides 0.3 to 0.5 ml of conjugate from approx. 0.35 mg IgG; this quantity can be diluted 1:4,000 to 1:10,000 for ELISA applications.
Contents
Alkaline phosphatase from calf intestine lyophilizate from 0.5 ml.
Application
The reagent can be used for labeling water-soluble substances with reactive and accessible primary amino groups (e.g., peptides or proteins) with alkaline phosphatase for use in analytical methods.
It is particularly suitable for the coupling of antibodies with alkaline phosphatase, as the resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.
The enzyme can be used without pre-activation for the labeling of Ig, Ig Fab, and Ig F(ab′)2 fragments from rabbit, mouse, sheep, and goat.
It is particularly suitable for the coupling of antibodies with alkaline phosphatase, as the resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.
The enzyme can be used without pre-activation for the labeling of Ig, Ig Fab, and Ig F(ab′)2 fragments from rabbit, mouse, sheep, and goat.
Preparation Note
Working solution: Dissolving the lyophilizate in 0.5 ml double dist. Water produces an alkaline phosphatase concentration of 20 mg/ml.
Storage conditions (working solution): The reconstituted solution is stable for 5 days at 2 to 8 °C. The solution can be aliquoted and shock frozen at -70 °C or below, and then stored at -15 to -25 °C.
Storage conditions (working solution): The reconstituted solution is stable for 5 days at 2 to 8 °C. The solution can be aliquoted and shock frozen at -70 °C or below, and then stored at -15 to -25 °C.
Analysis Note
Purity: >95% (HPLC)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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