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11579681001

Roche

RNase, DNase-free, High Concentration

from bovine pancreas

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Synonym(s):
Rnase

biological source

bovine pancreas

Quality Level

100

form

solution

specific activity

≥30 U/mg

packaging

pkg of 1 mg (10 mg/ml)

manufacturer/tradename

Roche

concentration

10 mg/mL

Related Categories

General description

A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.

Application

High concentration, DNase-free RNase has been used for:
  • Genomic DNA isolation
  • Isolation of plasmid DNA
  • Degradation of RNA for flow cytometry

Quality

Absence of DNase activity tested according to the current Quality Control procedures.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Preparation Note

Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0

Other Notes

For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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BET Bromodomain Inhibition as a Therapeutic Strategy to Target c-Myc
Jake E, et al.
Cell, 146(6) (2011)
Diana Pacheco Sousa et al.
STAR protocols, 3(4), 101916-101916 (2023-01-04)
This protocol describes the synthesis and characterization of gold nanoparticle-based nanobeacons as a theranostic strategy for the recognition, detection, and inhibition of miRNA and mRNA. This system is designed for an in vitro evaluation of a sequence's silencing potential and later
Alba Font-Tello et al.
Nature protocols, 15(8), 2503-2518 (2020-06-28)
Fixed-tissue ChIP-seq for H3K27 acetylation (H3K27ac) profiling (FiTAc-seq) is an epigenetic method for profiling active enhancers and promoters in formalin-fixed, paraffin-embedded (FFPE) tissues. We previously developed a modified ChIP-seq protocol (FiT-seq) for chromatin profiling in FFPE. FiT-seq produces high-quality chromatin
Artyom A Alekseyenko et al.
Current protocols in molecular biology, 109, 21-21 (2015-01-07)
In order to understand how chromatin complexes function in the nucleus, it is important to obtain a comprehensive picture of their protein, DNA, and RNA components, as well as their mutual interactions. This unit presents a chromatin cross-linking approach (BioTAP-XL)

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