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Merck
CN

11585029001

Roche

NBT

crystals, pkg of 5 g

Synonym(s):

Nitrotetrazolium Blue chloride, 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
298-83-9
Molecular Weight:
817.64
PubChem Substance ID:
329749491
UNSPSC Code:
41116100
Beilstein/REAXYS Number:
4115923
MDL number:
MFCD00012159
NACRES:
NA.54

Key Documents

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Product Name

NBT, crystals, pkg of 5 g

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

description

4-Nitro blue tetrazolium chloride, crystals

form

crystals

packaging

pkg of 5 g

manufacturer/tradename

Roche

storage temp.

2-8°C

Quality Level

100

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Preparation Note

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Analysis Note

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].
Performance-controlled in the LDH assay.

Application

  • Colony and plaque hybridization
  • Immunohistocytochemistry
  • In situ hybridization
  • Northern blot
  • Southern blot
  • Western blot
NBT has been used in in situ hybridization.

General description

NBT is soluble in water, aqueous buffers, and dimethylformamide.
Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
70503831
70503822
61288023
61288022
61288021

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Infection and nitroblue-tetrazolium reduction by neutrophils: A diagnostic aid.
Park B H, et al.
Lancet, 292(7567), 532-534 (1968)
D Liebhart et al.
Journal of comparative pathology, 135(4), 237-242 (2006-10-31)
For use in an in-situ hybridization method, three probes (HM, TR and BL) were designed to hybridize, respectively, with (1) Histomonas meleagridis, (2) Tetratrichomonas gallinarum, and (3) a broad range of micro-organisms, including Blastocystis spp. Mono-eukaryotic cultures were used to
Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction.
Berridge M V, et al.
Biotechnology Annual Review, 11, 127-152 (2005)
Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain.
Trinh L A, et al.
Biotechniques, 42(6), 756-759 (2007)
Wanlong Su et al.
Frontiers in plant science, 11, 568411-568411 (2021-02-09)
Salt stress is an adverse environmental factor for plant growth and development. Under salt stress, plants can activate the selective autophagy pathway to alleviate stress. However, the regulatory mechanism of selective autophagy in response to salt stress remains largely unclear.
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Protocols

NBT Protocol

NBT Protocol

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