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About This Item
UNSPSC Code:
41105500
NACRES:
NA.54
form
solution
usage
sufficient for 20 reactions
packaging
pkg of 40 μL
manufacturer/tradename
Roche
technique(s)
Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable
storage temp.
−20°C
General description
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Application
Biochem/physiol Actions
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Preparation Note
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Analysis Note
The mix is function tested in combination with the DIG RNA Labeling Kit.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
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