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11699075001

Roche

Nylon Membranes for Colony and Plaque Hybridization

Synonym(s):

membrane

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About This Item

UNSPSC Code:
41105339
NACRES:
NA.54
Packaging:
pkg of 50 discs (82 mm diameter)
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packaging

pkg of 50 discs (82 mm diameter)

manufacturer/tradename

Roche

disc diam.

82 mm

storage temp.

20-25°C

General description

Nylon membrane is a circular, uncharged, microporous, amphoteric, ultra-pure nylon 66 membrane on a polyester support. It has a durable structure with consistent transfer rate.
Surface Properties: Pore size of 1.2 μm. Surface contains 50% amino and 50% carboxyl groups.

Application

  • The membrane discs are specifically suited for:Nonradioactive screening of phage or cosmid libraries with digoxigenin-labeled probes followed by detection with highly sensitive chemiluminescent or chromogenic substrates
  • Hybridization with biotinylated or radioactive probes
  • It has been used in the colony immunoblotting technique.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Certificates of Analysis (COA)

Lot/Batch Number
88077800
64468700
46981100
20496300
46981500

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K P Karey et al.
Analytical biochemistry, 178(2), 255-259 (1989-05-01)
Western blotting of low molecular weight (Mr) acidic and basic isoelectric point (pI) proteins was studied to optimize detection sensitivities. Radioiodinated epidermal growth factor (EGF, Mr 6045, pI 4.4), transforming growth factor type alpha (TGF alpha, Mr 5623, pI 6.8)

Global Trade Item Number

SKUGTIN
1169907500104061838704672