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11699105001

Roche

PCR Buffer Without MgCl₂, 10x concentrated

Name: PCR Buffer Without MgCl<SUB>2</SUB>, 10x concentrated
Brand: ROCHE

solution, pkg of 3 × 1 mL

Synonym(s):

buffer

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About This Item

UNSPSC Code:

41105600

NACRES:

NA.54

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Properties

form

solution

packaging

pkg of 3 × 1 mL

manufacturer/tradename

Roche

technique(s)

PCR: suitable, whole genome amplification: suitable

storage temp.

−20°C

Description

General description

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Application

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

Physical form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Other Notes

For general laboratory use.

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

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