form
suspension
specific activity
~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
packaging
vial of 2 mL (10127833001), vial of 5 mL (10127841001)
manufacturer/tradename
Roche
concentration
5 mg/mL
impurities
<0.1% LDH, <5% MDH
optimum pH
6.2-6.9(for reduction), 7-9(for oxidation)
shipped in
wet ice
storage temp.
2-8°C
General description
Application
3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Stabilizers: NADH, Ca2+
Analysis Note
Contaminants: <0.1% LDH, <5% MDH
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
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