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AGRLE-RO

Roche

Agarose LE

low electroendosmosis

Synonym(s):

agarose

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
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biological source

algae (seaweed)

form

powder

packaging

pkg of 100 g (11685660001), pkg of 500 g (11685678001)

manufacturer/tradename

Roche

EEO

0.05 -0.13

transition temp

gel point 36 °C ±1.5

gel strength

>=2,500 g/cm2

foreign activity

Dnase, none detected, Rnase, none detected

shipped in

ambient

storage temp.

20-25°C

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

  • analyzing fragments between 0.2 and 15 kb
  • analysis of PCR products
  • examination of restriction endonucleases
  • digests of plasmid, cosmid, and λ phage DNA
  • electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
  • in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Analysis Note

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

No data available

Flash Point (°C)

No data available

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