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BCIPP-RO

Roche

BCIP

99% (HPLC), powder, solubility: in DMF, soluble

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Synonym(s):
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt
Empirical Formula (Hill Notation):
C8H6BrClNO4P · C7H9N
CAS Number:
6578-06-9
Molecular Weight:
433.62
MDL number:
MFCD00040636
PubChem Substance ID:
329774194

description

5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt

Quality Level

100

Assay

99% (HPLC)

form

powder

mol wt

Mr 326.44 (BCIP)
Mr 433.6 (BCIP toluidine salt)

packaging

pkg of 1 g (11585002001)
pkg of 250 mg (10760994001)

manufacturer/tradename

Roche

solubility

DMF: soluble

storage temp.

2-8°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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General description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Quality

Performance test: incubation with AP

Substrates

Histochemical substrate for alkaline phosphatase.

Analysis Note

Absorption: Absorption maximum in the visible light is not determined.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H312 - H315 - H319 - H335

Hazard Classifications

Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

136.4 °F

Flash Point(C)

58 °C

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David G Simmons et al.
Developmental biology, 304(2), 567-578 (2007-02-10)
Trophoblast giant cells (TGCs) are the first terminally differentiated subtype to form in the trophoblast cell lineage in rodents. In addition to mediating implantation, they are the main endocrine cells of the placenta, producing several hormones which regulate the maternal

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