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BSAV-RO

Roche

Bovine Serum Albumin

≥98.5%, New Zealand origin

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Synonym(s):
BSA, Bovine Serum Albumin Fraction V, Bovine albumin, bovine serum

biological source

bovine

Quality Level

Assay

≥98.5%

form

lyophilized

mol wt

Mr =68 kDa

packaging

pkg of 1 kg (10735108001)
pkg of 100 g (10735086001)
pkg of 50 g (10735078001)
pkg of 500 g (10735094001)

manufacturer/tradename

Roche

origin

New Zealand origin

technique(s)

ELISA: suitable
Southern blotting: suitable
western blot: suitable

impurities

<0.0003 Lithium (Flame photometry)
<0.001 Iron (bathophenanthraline)
<0.002 P (inorganic)
<0.003 Heavy metals (as Pb)
<0.003 Magnesium (xylidyl blue)
<0.005 Glycerol (enzyme)
<0.006 potassium (Flame photometry)
<0.02 Calcium ( o-cresolphthalein)
<0.05 glucose (enzyme)
<0.1 L-lactate
<0.15 chloride (mercurom)
<0.5 Sodium (flame photometry)
<100 microorganisms (organism/g)
<5 water

absorption

≤0.4 at 550 nm

UniProt accession no.

foreign activity

Microorganisms <100 (Organism/g)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

For standard applications.

Application

Bovine Serum Albumin Fraction V is suitable for standard applications such as:
  • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
  • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
  • Reducing agent
  • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
  • Media supplement for media that require addition of protein
  • Carrier protein
  • Standard protein in gel electrophoresis and protein determination

Quality

Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

Preparation Note

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Francis K Y Siu et al.
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Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor.
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