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CSPD-RO

Roche

CSPD ready-to-use

Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate

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Synonym(s):
ready-to-use, cspd

Quality Level

100

Assay

>98% (HPLC)

form

solution

mol wt

Mr 461

packaging

pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)

manufacturer/tradename

Roche

storage temp.

2-8°C

Related Categories

General description

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

Application

CSPD ready-to-use has been used for:
  • the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
  • colony and plaque hybridization
  • gel shift assay
  • sequencing
It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

Features and Benefits

  • Save time with a fast, highly sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
  • Reuse the substrate by filtering and storing in sodium azide.

Physical form

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

does not flash

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C S Martin et al.
BioTechniques, 18(5), 908-913 (1995-05-01)
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Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that

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