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Roche

Fibronectin (pure)

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

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Synonym(s):
Fibronectin
MDL number:
MFCD00131062

biological source

human plasma

Quality Level

100

sterility

non-sterile; 0.2 μm filtered

Assay

>95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

mol wt

440 kDa

packaging

pkg of 1 mg (11051407001)
pkg of 5 mg (11080938001)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02751

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Specificity

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

Sequence

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Preparation Note

Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Roberta Bulla et al.
Nature communications, 7, 10346-10346 (2016-02-03)
Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Benjamin N Rollo et al.
F1000Research, 4, 113-113 (2015-06-13)
The avian enteric nervous system (ENS) consists of a vast number of unusually small ganglia compared to other peripheral ganglia. Each ENS ganglion at mid-gestation has a core of neurons and a shell of mesenchymal precursor/glia-like enteric neural crest (ENC)
Fibronectin at a glance.
Roumen Pankov et al.
Journal of cell science, 115(Pt 20), 3861-3863 (2002-09-24)

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Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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