biological source
yeast (Candida boidini)
Quality Level
recombinant
expressed in E. coli
form
lyophilized
specific activity
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
mol wt
74 kDa
packaging
pkg of 250 U (10837016001)
pkg of 80 U (10244678001)
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
off-white
optimum pH
7.5-8.5
solubility
water: soluble 10 g/L
suitability
suitable for gel electrophoresis
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.0500%
LDH <0.0500%
MDH <0.1000%
storage temp.
2-8°C
Related Categories
General description
Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Application
Most widely used in cofactor recycling systems for NADH.
Biochem/physiol Actions
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Preparation Note
Store dry
Analysis Note
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Katja Schirwitz et al.
Protein science : a publication of the Protein Society, 16(6), 1146-1156 (2007-05-26)
The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH).
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
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