biological source
yeast (Candida boidini)
recombinant
expressed in E. coli
form
lyophilized
specific activity
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
mol wt
74 kDa
packaging
pkg of 250 U (10837016001), pkg of 80 U (10244678001)
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
off-white
optimum pH
7.5-8.5
solubility
water: soluble 10 g/L
suitability
suitable for gel electrophoresis
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.0500%, LDH <0.0500%, MDH <0.1000%
storage temp.
2-8°C
General description
Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Application
Most widely used in cofactor recycling systems for NADH.
Biochem/physiol Actions
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Preparation Note
Store dry
Analysis Note
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
常规特殊物品
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10244678001 | 04061838669636 |
| 10837016001 | 04061838683885 |