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G418-RO

Roche

G-418 Solution

Name: G-418 Solution
Brand: ROCHE

solution, =98% (TLC), suitable for transfection

Synonym(s):

G 418 disulfate salt solution, O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, neomycin, Antibiotic G418

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

CAS Number:

108321-42-2

Molecular Weight:

692.71

MDL number:

MFCD00058314

UNSPSC Code:

12352200

PubChem Substance ID:

329799925

NACRES:

NA.28

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Properties

assay

98% (TLC)

form

solution

mol wt

692.7 Da

packaging

pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g]), pkg of 20 mL (04727878001 [equivalent to 1 g])

manufacturer/tradename

Roche

concentration

50 mg/mL

technique(s)

transfection: suitable

antibiotic activity spectrum

protozoa

mode of action

protein synthesis | inhibits

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

General description

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Application

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neo^r) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Features and Benefits

Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
Function tested in a cell culture assay - ensures effectiveness.
Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
Cost effective, since the selected colonies are stable.

Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Preparation Note

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Analysis Note

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c