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G6PDHII-RO

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade II, from yeast

Synonym(s):

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

UNSPSC Code:
12352204
EC Number:
1.1.1.49 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)
Biological source:
yeast
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biological source

yeast

form

suspension

specific activity

~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)

packaging

pkg of 2 mL (10127671001 [5 mg/mL]), pkg of 5 mL (10737232001 [5 mg/mL])

manufacturer/tradename

Roche

optimum pH

9.2

pH range

5-11

shipped in

wet ice

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.

Application

Glucose-6-Phosphate Dehydrogenase (G6P-DH) is a component of cofactor recycling systems for NADPH. Additional reagent glucose-6-phosphate.
It was used in enzymatic determination of C6 phosphorylation of glycogen.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.0

Preparation Note

Activator: Mg2+ (5 to 10 mM)

Analysis Note

Contaminants: <0.001% CK, <0.05% GR, <0.01% 6-PGDH and PGluM each, <0.02% HK, <0.002% PGI

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
10986926
10986925
77783020
48693336
48693334

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Anna A DePaoli-Roach et al.
The Journal of biological chemistry, 290(2), 841-850 (2014-11-25)
Glycogen is a branched polymer of glucose that acts as an energy reserve in many cell types. Glycogen contains trace amounts of covalent phosphate, in the range of 1 phosphate per 500-2000 glucose residues depending on the source. The function

Global Trade Item Number

SKUGTIN
1012767100104061838667397
1073723200104061838675019