Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Biological source:
yeast
Concentration:
3.21 mg/mL
biological source
yeast
form
suspension
mol wt
dimer 110,000 Da
packaging
pkg of 10 mL (10737275001 [30 mg]), pkg of 5 mL (10127825001 [15 mg])
manufacturer/tradename
Roche
concentration
3.21 mg/mL
technique(s)
activity assay: suitable
color
white
pH
5.5-6.5
solubility
water: miscible
suitability
suitable for UV spectrophotometry and general use
application(s)
life science and biopharma
storage temp.
2-8°C
General description
Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Application
Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
Other Notes
EC 2.7.1.1 & 1.1.1.49
For life science research only. Not for use in diagnostic prodcedures.
One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
K Gottlob et al.
Genes & development, 15(11), 1406-1418 (2001-06-08)
The serine/threonine kinase Akt/PKB is a major downstream effector of growth factor-mediated cell survival. Activated Akt, like Bcl-2 and Bcl-xL, prevents closure of a PT pore component, the voltage-dependent anion channel (VDAC); intracellular acidification; mitochondrial hyperpolarization; and the decline in
Miguel Vázquez-Velasco et al.
Nutricion hospitalaria, 32(6), 2718-2724 (2015-12-17)
Type 2 diabetes is a very prevalent chronic disease. Among dietary factors for its prevention and treatment, interest has grown in satiating fibre (konjac glucomannan) and spirulina. Our previous studies suggest that glucomannan itself and/or in conjunction to spirulina displayed
H Pelicano et al.
Oncogene, 25(34), 4633-4646 (2006-08-08)
Most cancer cells exhibit increased glycolysis and use this metabolic pathway for generation of ATP as a main source of their energy supply. This phenomenon is known as the Warburg effect and is considered as one of the most fundamental
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10127825001 | 04061838667502 |
| 10737275001 | 04061838675026 |