Sign In to View Organizational & Contract Pricing
Select a Size
About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
usage
sufficient for 100 reactions (KK7351)
sufficient for 100 reactions
sufficient for 500 reactions (KK7352)
sufficient for 500 reactions
Quality Level
shelf life
≤18 mo.
feature
dNTPs included
hotstart
manufacturer/tradename
Roche
technique(s)
PCR: suitable
genotyping: suitable
input
crude DNA
storage temp.
−20°C
General description
KAPA HotStart® Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA from mouse tissue in less than 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA HotStart Mouse Genotyping Kit is designed for rapid extraction and amplification of DNA from mouse tissue.
Application
KAPA HotStart® Mouse Genotyping Kit has been used for:
- extraction and amplification from mouse tail, ear, and toe tissue
- extraction and amplification of DNA from other animal tissue
Features and Benefits
Increased throughput, turnaround time and reliability
Outperforms crude extraction methods and commercial kits
Quick Notes:
- PCR-ready DNA generated in as little as 15 minutes.
- Minimal handling, thereby reducing the risk of sample loss or contamination.
- Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format.
- Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents.
Outperforms crude extraction methods and commercial kits
- Increased turnaround times
- Increased specificity
- Decreased cost
Quick Notes:
- Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes.
- Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0–8.5), and use 1 μL of DNA per 25 μL reaction.
- Extracts are stable at -20°C for >6 months.
- KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl2 (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
- Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
- Use 15 sec annealing time, with 15 sec extension time for amplicons <1 kb, or 15–30 sec/kb extension for longer amplicons.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).
Analysis Note
All components contained with KAPA HotStart Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo and endonuclease activities and meet strict requirements with respect to DNA contamination.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
Kit Components Only
Product No.
Description
- KAPA Express Extract Enzyme 1 U/µL
- 10X KAPA Express Extract Buffer
- 2X KAPA2G Fast HotStart® Genotyping Mix with dye
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
5-HT2A receptor deficiency alters the metabolic and transcriptional, but not the behavioral, consequences of chronic unpredictable stress.
Jaggar M, et al.
Neurobiology of stress, 7, 89-102 (2017)
Discovery of a new path for red blood cell generation in the mouse embryo.
Pinheiro I, et al.
bioRxiv, 309054-309054 (2018)
Namit Kumar et al.
Development (Cambridge, England), 146(5) (2019-02-13)
Lineage-restricted transcription factors, such as the intestine-specifying factor CDX2, often have dual requirements across developmental time. Embryonic loss of CDX2 triggers homeotic transformation of intestinal fate, whereas adult-onset loss compromises crucial physiological functions but preserves intestinal identity. It is unclear
Stefanie Zeitler et al.
Cells, 9(11) (2020-11-22)
: The acid sphingomyelinase (ASM)/ceramide system exhibits a crucial role in the pathology of major depressive disorder (MDD). ASM hydrolyzes the abundant membrane lipid sphingomyelin to ceramide that regulates the clustering of membrane proteins via microdomain and lipid raft organization.
Maria Saliakoura et al.
Oncogene, 39(14), 2948-2960 (2020-02-09)
Enhanced prostaglandin production promotes the development and progression of cancer. Prostaglandins are generated from arachidonic acid (AA) by the action of cyclooxygenase (COX) isoenzymes. However, how cancer cells are able to maintain an elevated supply of AA for prostaglandin production
Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Related Content
默克致力于解决生命科学最棘手的挑战问题。现在，我们进一步扩展了罗氏产品的经销协议，借助高品质产品和全球知名品牌来增强自身分子生物学产品线，为您带来当今市面上更有效的生命科学工具，默克产品优势灵敏度更高，合成速度更快，合成能力更强
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service