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NAD100-RO

Roche

NAD

Name: NAD
Brand: ROCHE

Grade I, free acid

Synonym(s):

β-Nicotinamide adenine dinucleotide hydrate, β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₂₇N₇O₁₄P₂ · xH₂O

CAS Number:

53-84-9

Molecular Weight:

663.43 (anhydrous basis)

UNSPSC Code:

41106305

PubChem Substance ID:

329818699

MDL number:

MFCD00036253

NACRES:

NA.54

eCl@ss:

39200202

Assay:

100% (dry weight)

Form:

lyophilized

Storage temp.:

2-8°C

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assay

100% (dry weight)

form

lyophilized

mol wt

M_r 663.4

packaging

pkg of 1 g (10127965001), pkg of 5 g (10127973001)

manufacturer/tradename

Roche

color

off-white

pH

~3.0 (50 mg/mL in water)

λ_max

260 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

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General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

Biochem/physiol Actions

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

Electron acceptor

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Features and Benefits

Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)

Packaging

Packaged by solid weight.

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Analysis Note

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Other Notes

For life science research only. Not for use in diagnostic procedures.

This is the common form of NAD.

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Helicobacter pylori porCDAB and oorDABC genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to NADP.

N J Hughes et al.

Journal of bacteriology, 180(5), 1119-1128 (1998-03-12)

Helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953-3959, 1995)

The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction

Fernandes PM, et al.

The Biochemical Journal, 476(2), 179-191 (2019)

Kinetic mechanism of human class IV alcohol dehydrogenase functioning as retinol dehydrogenase.

Chu-Fang Chou et al.

The Journal of biological chemistry, 277(28), 25209-25216 (2002-05-09)

Molecular genetic studies have indicated that alcohol dehydrogenase may be involved in the synthesis of retinoic acid, a hormonal molecule regulating diverse cellular functions at the transcriptional level. Class IV alcohol dehydrogenase (ADH) has been reported to be the most

SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation

Sarikhani M, et al.

eLife, 7(1), e32952-e32952 (2018)

Extracellular beta-nicotinamide adenine dinucleotide (beta-NAD) promotes the endothelial cell barrier integrity via PKA-and EPAC1/Rac1-dependent actin cytoskeleton rearrangement

Umapathy NS, et al.

Journal of Cellular Physiology, 223(1), 215-223 (2010)

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Global Trade Item Number

SKU	GTIN
10127965001	04061838667588
10127973001	04061838251756

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