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About This Item
NACRES:
NA.55
UNSPSC Code:
41106300
Assay:
>99% (HPLC)
Form:
liquid
Solubility:
water: miscible
Storage temp.:
−20°C
grade
Molecular Biology
assay
>99% (HPLC)
form
liquid
shelf life
≤18 mo.
packaging
pkg of 5 mL (KK1041), pkg of 500 μL (KK1017)
manufacturer/tradename
Roche
impurities
Bacterial contamination assay, passes test, DNase, none detected, Endonuclease contamination assay, passes test, Exonuclease contamination assay, passes test, Human contamination assay, passes test, RNase, none detected, nicking activity, none detected, phosphatase, none detected, protease, none detected
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C
General description
KAPA dinucleotide triphosphates (dNTPs) provide high-quality, molecular, premium grade dinucleotide phosphates (dNTPs) mainly used for polymerase chain reaction (PCR).
Application
KAPA dNTP Mix has been used in:
- Standard PCR
- High fidelity PCR
- Quantitative PCR
- End-repair or A-tailing
- cDNA synthesis
- DNA sequencing
- DNA labeling
- Microarrays
Features and Benefits
- KAPA dNTPs are available as individual dNTPs or as a ready-to-use dNTP Mix.
- KAPA dNTP Mix is supplied with KAPA Taq, KAPA2G Fast, KAPA2G Robust, KAPA LongRange and KAPA HiFi PCR Kits.
- KAPA dNTPs are subjected to strict quality control to ensure high performance, particularly with B-family polymerases that are sensitive to poisoning by uracil.
- Compatible with any commercially available DNA polymerases.
Preparation Note
Always ensure that the product KAPA dNTP Mix has been fully thawed and mixed before use. KAPA dNTPs may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the sample has been handled carefully and not contaminated, the samples are not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that storing the reagents at temperatures above -20°C makes them more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
Analysis Note
KAPA dNTPs are >99% pure by HPLC and are free of DNase, RNase, protease, phosphatase, and nicking activity. All kit components are subjected to stringent functional quality control, and meet strict requirements with respect to DNA contamination.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Mixture of dATP, dCTP, dGTP and dTTP 10 mM each
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| KK1041 | 04061837905353 |
| KK1017 | 04061837905346 |