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PCRH20-RO

Roche

Water, PCR Grade

liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])

Synonym(s):

Water, PCR Grade

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About This Item

UNSPSC Code:
12191602
EC Number:
231-791-2
NACRES:
NA.21

Key Documents

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Product Name

Water, PCR Grade, liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])

form

liquid

packaging

pkg of 100 mL (03315843001 [4 x 25 ml])
pkg of 25 mL (03315932001 [25 x 1 ml])
pkg of 25 mL (03315959001 [1 x 25 ml])

manufacturer/tradename

Roche

dilution

(for analytical testing)

storage temp.

−20°C

Quality Level

100

Related Categories

Analysis Note

Function test: Each lot of Water, PCR Grade is tested in PCR (amplification of a 0.5 kb target from 0.01 ng λDNA). When the PCR product is analyzed by gel electrophoresis, a clear, defined band must be seen on the gel.
Absence of nucleases: The water is tested with various nucleic acids that could serve as nuclease substrates. No endonucleases, ribonucleases or nicking activity are detected according to the current Quality procedures.

Application

This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality water is needed. All of the LightCycler® kits and some of the amplification products — available from Roche Applied Science — contain this Water, PCR Grade.

General description

Water, PCR Grade is specially purified, double-distilled, deionized, and autoclaved. The production process ensures that even according to Roche ′s strict Quality Control procedures the water does not contain detectable amounts of nucleic acid. It is routinely tested in PCR with conventional thermal cyclers and with LightCycler® Instruments.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Storage conditions (working solution): Store a working solution for a short period of time (e.g. 1 week) at 2 to 8 °C.

Legal Information

LightCycler is a registered trademark of Roche

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
53000600
52127400
51254700
50712200
50313400

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Y Chaïbou et al.
Medecine et maladies infectieuses, 44(3), 117-122 (2014-03-13)
We had for aim to determine the epidemiology of meningeal and lung invasive infections due to Streptococcus pneumoniae in Burkina Faso. We screened for S. pneumoniae with the usual bacteriology techniques and with real time polymerase chain reaction (rt-PCR) in 7917
Eric K Sackmann et al.
Journal of laboratory automation, 21(1), 166-177 (2015-09-06)
Acoustic liquid handling uses high-frequency acoustic signals that are focused on the surface of a fluid to eject droplets with high accuracy and precision for various life science applications. Here we present a multiwell source plate, the Echo Qualified Reservoir
Bruce Humphrey et al.
PloS one, 10(7), e0132954-e0132954 (2015-07-15)
More than two decades after its discovery, contaminant microbial DNA in PCR reagents continues to impact the sensitivity and integrity of broad-range PCR diagnostic techniques. This is particularly relevant to their use in the setting of human sepsis, where a

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