Quality Level
usage
sufficient for 100 reactions
20 μL sufficient for 100 reactions
sufficient for 500 reactions
20 μL sufficient for 500 reactions
shelf life
≤12 mo.
feature
dNTPs included
hotstart
packaging
kit of 1 mL (100 x 20 μL rxn; KK4300)
kit of 5 mL (500 x 20 μL rxn; KK4301)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
qPCR: suitable
input
crude DNA
detection method
probe-based
storage temp.
−20°C
Related Categories
General description
- Direct qPCR from crude blood, tissue, and plant extracts
- Sample-to-Cq workflows in <1 hour
- High efficiency for accurate, reproducible, and sensitive results
- Superior tolerance to carry-over inhibitors
- Multiplex compatibility with crude extracts
Application
- GMO testing
- Mouse transgenics
- SNP genotyping
- Food/water pathogen detection
- Infectious disease research
- Cancer research
- DNA quantification
- Quantitative polymerase chain reaction (qPCR)
- Digital droplet PCR
- For amplification of templates directly from cDNA synthesis reactions
Features and Benefits
- Eliminate the time and cost of sample purification by amplifying directly from crude samples
- Analyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soil
Generate accurate and reproducible results:
- Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection
- Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation
Break through high levels of qPCR inhibitors:
- Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples
- Convert purified DNA assays to crude workflows without observable Cq delays
Multiplex crude samples efficiently:
- Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts
- Maximize data collection from precious samples, increase throughput, and reduce costs
Quick Notes:
- This kit contains the KAPA3G HotStart DNA Polymerase enzyme, enabling probe-based qPCR for both routine and challenging sample types.
- Initial denaturation of 3 min at 98°C is recommended to ensure complete denaturation of complex target DNA. A 5-min denaturation time may be required for some crude samples.
- For two-step cycling, use a 20-sec combined annealing/extension/data acquisition at 60°C as a first approach.
- A 10-sec annealing/extension/data acquisition time may be used with most assays, but this must be determined empirically.
- For crude samples, the amount of sample in the reaction may be reduced to improve performance, but this must be determined empirically.
Quality
Preparation Note
Other Notes
Kit Components Only
- KAPA3G HotStart® DNA Polymerase
- dNTPs (including dUTP)
- MgCl2 4.5 mM at 1X
- ROX™ reference dye
- stabilizers
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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