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KAPA PROBE FAST

suitable for qPCR, 2 ×, Universal

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Synonym(s):
qPCR
NACRES:
NA.55

Quality Level

100

usage

sufficient for 100 reactions
 sufficient for 1000 reactions
 sufficient for 500 reactions
 sufficient for 5000 reactions

shelf life

≤12 mo.

feature

dNTPs included
hotstart

packaging

kit of 1 mL (100 x 20 μL rxn; KK4701)
kit of 10 mL (1000 x 20 μL rxn; KK4703)
kit of 5 mL (500 x 20 μL rxn; KK4702)
kit of 50 mL (5000 x 20 μL rxn; KK4715)

manufacturer/tradename

Roche

concentration

2 ×

technique(s)

qPCR: suitable

input

purified DNA

compatibility

for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7500
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™
for use with StepOne™
for use with StepOnePlus™

detection method

probe-based

storage temp.

−20°C

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General description

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).

Application

KAPA PROBE FAST may be used for:
  • Real-time quantitative methylation-specific PCR (qMSP)
  • Genotyping
  • Gene expression analysis
  • Multiplex qPCR and qRT-PCR
  • SNP genotyping/allelic discrimination
  • Microarray validation

Features and Benefits

Accurately discriminate between alleles:
  • Exceptional differentiation of heterozygous and homozygous alleles
  • Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:
  • Fast, high performance five-colored multiplex PCR
  • Similar abundance levels achieved with low copy number
  • Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:
  • Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:
  • These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
  • Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
  • For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
  • Do not exceed 25 μL volumes.

Quality

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Preparation Note

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Only

Product No.
Description
  • KAPA Taq HotStart® DNA Polymerase
  • 50X ROX™ High Reference Dye
  • 50X ROX™ Low Reference Dye
  • reaction buffer
  • dNTPs
  • MgCl2 5 mM

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Cell-free methylation markers with diagnostic and prognostic potential in hepatocellular carcinoma.
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