biological source
yeast
Quality Level
form
suspension
specific activity
~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
packaging
pkg of 1 mL (10127396001 [2 mg])
pkg of 1 mL (10128139001 [10 mg])
manufacturer/tradename
Roche
optimum pH
7.0-7.6
storage temp.
2-8°C
Related Categories
General description
Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.
Application
Isomerization of ketoses to aldoses.
Biochem/physiol Actions
Phosphoglucose isomerase (PGI) is specific for G6P (Km = 0.7 mM) and fructose-6-phosphate (F6P).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.
Analysis Note
Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
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