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ROAHA

Roche

Anti-HA (12CA5)

from mouse IgG2bκ

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Synonym(s):
antibody

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12CA5, monoclonal

Assay

>90% (HPLC and SDS-PAGE)

form

frozen liquid (11666606001)
lyophilized (11583816001)

packaging

pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])

manufacturer/tradename

Roche

concentration

5 mg/ml (11666606001)

isotype

IgG2bκ

epitope sequence

YPYDVPDYA

storage temp.

−20°C

Related Categories

General description

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.

Specificity

Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
  • Dot blots
  • Immunochemistry
  • Immunoprecipitation
  • Western blotting
Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).

Biochem/physiol Actions

Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.

Quality

Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 0.1 to 1 μg/ml
  • Immunfluorescence: 1-10 μg/ml
  • Western blot: 0.1 to 1 μg/ml

Reconstitution

11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

13 - Non Combustible Solids

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

常规特殊物品

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T1503
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-
25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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On the origin of the human influenza virus subtypes H2N2 and H3N2
Scholtissek C, et al.
Virology, 87(1), 13-20 (1978)
Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus.
Stevens J, et al.
Science, 312(5772), 404-410 (2006)
Yiwei He et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(40), e2212134119-e2212134119 (2022-09-27)
Eukaryotic chromosomes are organized into structural and functional domains with characteristic replication timings, which are thought to contribute to epigenetic programming and genome stability. Differential replication timing results from epigenetic mechanisms that positively and negatively regulate the competition for limiting
Jen-Liang Su et al.
Cancer research, 71(21), 6878-6887 (2011-09-14)
Gene therapy trials in human breast, ovarian, and head and neck tumors indicate that adenovirus E1A can sensitize cancer cells to the cytotoxic effects of paclitaxel in vitro and in vivo. Resistance to paclitaxel has been reported to occur in
Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.
Skehel J J and Wiley D C
Annual Review of Biochemistry, 69(1), 531-569 (2000)
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