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About This Item
NACRES:
NA.56
UNSPSC Code:
41106300
shelf life
≤18 mo.
feature
dNTPs included: no, hotstart: no
packaging
pkg of 250 U (KK1020), pkg of 2500 U (BK1004), pkg of 500 U (KK1022), pkg of 5000 U (BK1006)
manufacturer/tradename
Roche
technique(s)
PCR: suitable using : suitable, WGA
input
purified DNA
storage temp.
−20°C
General description
KAPA Taq is supplied in a 2X ReadyMix™ format. It comprises all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq DNA Polymerase comprises two reaction buffers (Buffer A and Buffer B) or a single buffer with loading dye. It aids convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers. KAPA Taq Buffer B is a Tris-potassium chloride buffer. KAPA Taq DNA Polymerase combines with any standard Taq buffer with a pH of 8.3 or higher.
Application
KAPA Taq PCR Kit may be used in:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
- Polymorphism genotyping
Biochem/physiol Actions
KAPA Taq DNA Polymerase is a single-subunit, wild-type Taq DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities. It does not have 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq associates with a proprietary antibody and inactivates the enzyme until the first denaturation step. Hence, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
Features and Benefits
High performance :
Quick Notes :
- Improved sensitivity, specificity and yields
- Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.
Quick Notes :
- KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
- The final MgCl2 concentration may need to be optimized to account for differences in buffer formulation.
- KAPA Taq Buffers contain MgCl2 at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields.Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
- The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.
Analysis Note
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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Kit Components Only
Product No.
Description
- KAPA Taq DNA Polymerase 5 U/µL
- 10X KAPA Taq Buffer with loading dye
- MgCl2 25 mM
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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