24-0940
Pepsin from porcine gastric mucosa
CP
Synonym(s):
Pepsin A, Pepsin from hog stomach
grade
CP
form
powder
mol wt
35 kDa
availability
available only in Japan
dilution
(for analytical testing)
UniProt accession no.
Gene Information
pig ... LOC396892(396892)
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Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Biochem/physiol Actions
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Chuan-Hsiao Han et al.
Food chemistry, 138(2-3), 923-930 (2013-02-16)
Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of
Mahmoud S Ali et al.
The Laryngoscope, 123(2), 434-439 (2012-10-17)
To assess if, as previously reported in the literature, bile acids inhibit pepsin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease. Prospective translational research study. A total of 78 patient's fasting gastric juice
M N James et al.
Nature, 319(6048), 33-38 (1986-01-02)
The only well-understood mechanism of zymogen activation is that of the serine proteinases, in which proteolytic cleavage leads to conformational changes resulting in a functional active site. A different mechanism is now unveiled by the crystal structure of pepsinogen. Salt
Martial Rey et al.
Molecular & cellular proteomics : MCP, 12(2), 464-472 (2012-12-01)
Studies of protein dynamics, structure and interactions using hydrogen/deuterium exchange mass spectrometry (HDX-MS) have sharply increased over the past 5-10 years. The predominant technology requires fast digestion at pH 2-3 to retain deuterium label. Pepsin is used almost exclusively, but
Minghuo Wu et al.
Chemical communications (Cambridge, England), 49(14), 1407-1409 (2013-01-17)
A simple, novel "one-pot" approach was developed for the preparation of organic-silica hybrid monoliths. Two clickable monoliths functionalized with either azido or alkynyl moieties were prepared. The clickable monoliths were further used for the fabrication of enzyme reactors via "click
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