00879
8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt
≥98.0% (TLC)
Synonym(s):
1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate
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About This Item
Empirical Formula (Hill Notation):
C18H9Na3O11S3
CAS Number:
Molecular Weight:
566.42
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
9538267
assay
≥98.0% (TLC)
mp
238-248 °C (dec.) (lit.)
solubility
DMF: soluble, DMSO: soluble, H2O: soluble, methanol: soluble
fluorescence
λex 460 nm; λem 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
[Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O
Application
A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).
suitable as fluorogenic substrate for esterases
Other Notes
Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths
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