0120216
Half Fraser Broth in Bags
For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance with ISO 11290, suitable for microbiology
别名:
Fraser broth primary enrichment
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关于此项目
NACRES:
NA.74
UNSPSC Code:
41106200
Quality Segment
agency
ISO 11290, according to ISO 11290
sterility
sterile
form
liquid
shelf life
limited shelf life, expiry date on the label
feature
ready-to-use
composition
acriflavin hydrochloride, 0.0125 g/L , ammonium ferric citrate, 0.5 g/L , casein peptone, 5 g/L , di-sodium phosphate dihydrate, 12.0 g/L , esculin, 1.0 g/L , lithium chloride, 3.0 g/L , meat extract, 5 g/L , meat peptone, 5 g/L , nalidixic acid, 0.010 g/L , potassium dihydrogen phosphate, 1.35 g/L , sodium chloride, 20 g/L , yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
final pH
7.2±0.2 (25 °C)
application(s)
agriculture
environmental
food and beverages
industrial qc
pathogen testing
sample preparation, microbiology
suitability
Listeria spp.
General description
Half Fraser broth is a selective enrichment medium designed to help the recovery of Listeria sp. This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.
Application
Half Fraser broth is used for the selective pre-enrichment and detection of Listeria sp. in food and environmental specimens.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable