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0120216

Half Fraser Broth in Bags

For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance with ISO 11290, suitable for microbiology

Synonym(s):

Fraser broth primary enrichment

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106200
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Quality Segment

agency

ISO 11290, according to ISO 11290

sterility

sterile

form

liquid

shelf life

limited shelf life, expiry date on the label

feature

ready-to-use

composition

acriflavin hydrochloride, 0.0125 g/L , ammonium ferric citrate, 0.5 g/L , casein peptone, 5 g/L , di-sodium phosphate dihydrate, 12.0 g/L , esculin, 1.0 g/L , lithium chloride, 3.0 g/L , meat extract, 5 g/L , meat peptone, 5 g/L , nalidixic acid, 0.010 g/L , potassium dihydrogen phosphate, 1.35 g/L , sodium chloride, 20 g/L , yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
industrial qc
pathogen testing
sample preparation, microbiology

suitability

Listeria spp.

General description

Half Fraser broth is a selective enrichment medium designed to help the recovery of Listeria sp. This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Application

Half Fraser broth is used for the selective pre-enrichment and detection of Listeria sp. in food and environmental specimens.

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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