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114367

Bromocresol Green sodium salt

90% (HPLC), ACS reagent, Dye content 90 %

Synonym(s):

3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C21H13Br4NaO5S
CAS Number:
62625-32-5
Molecular Weight:
720.00
NACRES:
NA.47
PubChem Substance ID:
24847167
UNSPSC Code:
12171500
EC Number:
263-657-4
MDL number:
MFCD00148898
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grade

ACS reagent

Quality Level

200

agency

suitable for SM 2310, suitable for SM 2320

assay

90% (HPLC)

form

powder or crystals

composition

Dye content, 90%

visual transition interval

3.8, yellow(Visual Interval Pass), 3.8-5.2, yellow to blue, 4.5, green, 5.4, blue(Visual Interval Pass)

mp

230 °C (dec.) (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1

InChI key

HEFSGAHJDGZCHA-UHFFFAOYSA-M

General description

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is suitable as a selective inhibitor in kidney physiology studies.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT1424
SHBS7607
SHBR8655
SHBR8725
SHBQ8322

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This page is intended to make it easier to find the consumables you need based on the analytical method you’re using. Methods included on this page come from the EPA, Standard Methods and ASTM.

Conn?s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine (10th ed.)
RW Horobin, JA Kiernan (Eds.)
Conn?s Biological Stains (2002)
A high-throughput colorimetric assay to measure the activity of glutamate decarboxylase
Yu, et al.
Enzyme and Microbial Technology, 49(3) (2011)
Azo-functionalized superparamagnetic Fe3O4 nanoparticles: an efficient adsorbent for the removal of bromocresol green from contaminated water
Saad, et al.
Royal Society of Chemistry Advances, 12 (2022)
View All Related Papers

Global Trade Item Number

SKUGTIN
114367-25G04061838701954
114367-5G04061838701961