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114375

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
115-40-2
Molecular Weight:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847168
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
MFCD00011681
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product line

BioReagent

Quality Level

200

assay

≥89.0% (HPLC)

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λmax

419 nm

ε (extinction coefficient)

≥22000 at 418-424 nm in methanol

suitability

suitable for indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Spectrophotometric determination of serum-albumin

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000538592
0000534393
0000538592
0000543304
0000543301

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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
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Global Trade Item Number

SKUGTIN
114375-25G04061838701978
114375-5G04061838701985