114391
Bromophenol Blue
ACS reagent
Synonym(s):
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
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About This Item
Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-086-2
Beilstein/REAXYS Number:
61698
MDL number:
grade
ACS reagent
Quality Level
form
powder
technique(s)
titration: suitable
visual transition interval
3.0-4.6, yellow to blue
mp
273 °C (lit.)
solubility
ethanol: 0.1%
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key
UDSAIICHUKSCKT-UHFFFAOYSA-N
General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ibrahim Alkatout et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(1), 69-79 (2014-06-21)
The prognosis of pancreatic neuroendocrine tumors is related to size, histology and proliferation rate. However, this stratification needs to be refined further. We conducted a proteome study on insulinomas, a well-defined pancreatic neuroendocrine tumor entity, in order to identify proteins
Stephanie L Smith-Roe et al.
Oncotarget, 6(2), 732-745 (2014-12-30)
SWI/SNF complexes utilize BRG1 (also known as SMARCA4) or BRM (also known as SMARCA2) as alternative catalytic subunits with ATPase activity to remodel chromatin. These chromatin-remodeling complexes are required for mammalian development and are mutated in ~20% of all human
Ákos Kenéz et al.
PloS one, 10(5), e0127208-e0127208 (2015-05-16)
Dairy cows mobilize large amounts of body fat during early lactation to overcome negative energy balance which typically arises in this period. As an adaptation process, adipose tissues of cows undergo extensive remodeling during late pregnancy and early lactation. The
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 114391-5G | 04061835570195 |
| 114391-25G | 04061835570188 |