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Bromothymol Blue

Name: Bromothymol Blue
Brand: SIAL

ACS reagent, Dye content 95 %

Synonym(s):

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Empirical Formula (Hill Notation):

C₂₇H₂₈Br₂O₅S

CAS Number:

76-59-5

Molecular Weight:

624.38

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24847171

EC Number:

200-971-2

Beilstein/REAXYS Number:

373934

MDL number:

MFCD00005872

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Properties

grade

ACS reagent

Quality Segment

200

form

powder

composition

Dye content, 95%

color

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C ((392 - 396 °F) - lit.), 200-202 °C (lit.)

λ_max

420 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol, ≥8000 at 326-332 nm in methanol, ≥9000 at 279-285 nm in methanol

storage temp.

room temp

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

Description

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:

as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
for the demonstration of fungal hyphae within plant roots
in the assessment of the soluble acidity within single rice grains
use in an agar gel medium for enumeration of Bacillus cereus
as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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